Job Description

Requisition Number 07872
Department Name: Chemistry - FL
Position Type: Full Time
Position Title Postdoctoral Associate- Shen Lab
Position Summary:

A Postdoctoral Research Associate position in X-ray crystallography to elucidate the structures and functions of proteins from diverse natural product biosynthetic machineries is AVAILABLE IMMEDIATELY. Current research in the Shen lab centers on the chemistry, biochemistry and genetics of natural product biosynthesis in actinobacteria. By asking what reactions are available in nature, what are the enzymatic mechanisms of these reactions, how these reactions are linked to produce complex structures, what are the regulatory mechanisms of these pathways, we aim to translate the fundamental understanding of natural product biosynthesis into innovative strategies for natural product discovery and manipulate nature’s biosynthetic machinery for the discovery and development of new drugs (https://www.scripps.edu/faculty/shen/).

Selected publications on structures and functions of proteins from natural product biosynthetic machineries:

(1)Lohman, J.R.; Ma, M.; Osipiuk, J.; Nocek, B.; Kim, Y.; Chang, C.; Cuff, M.; Mack, J.; Bigelow, L.; Li, H.; Endres, M.; Babnigg, G.; Joachimiak, A.; Phillips, G.N. Jr.; Shen, B. (2015) Structural and evolutionary relationships of "AT-less" type I polyketide synthase ketosynthases. Proc. Natl. Acad. Sci. U.S.A. 112:12693-12698.
(2)Rudolf, J. D., Dong, L., Cao, H., Hatzos-Skintges, C., Osipiuk, J., Endres, M., Chang, C.-Y., Ma, M., Babnigg, G., Joachimiak, A., Phillips Jr., G. N., Shen, B. (2016). Structure of the ent-copalyl diphosphate synthase PtmT2 from Streptomyces platensis CB00739, a bacterial type II diterpene synthase. J. Am. Chem. Soc. 138:10905-10915.
(3)Chang, C.-Y.; Lohman, J. R.; Cao, H.; Tan, K.; Rudolf, J. D.; Ma, M.; Xu, W.; Bingman, C. A.; Yennamalli, R. M.; Bigelow, L.; Babnigg, G.; Yan, X.; Joachimiak, A.; Phillips, G.N. Jr.; Shen, B. (2016) Crystal structures of SgcE6 and SgcC, the two-component monooxygenase that catalyzes hydroxylation of a carrier protein-tethered substrate in biosynthesis of the enediyne antitumor antibiotic C-1027 in Streptomyces globisporus. Biochemistry. 55:5142-5154.
(4)Chang, C.-Y., Lohman, J. R., Huang, T., Michalska, K., Bigelow, L., Rudolf, J. D., Jedrzejczak, R., Yan, X., Ma, M., Babnigg, G., Joachimiak, A., Phillips Jr., G. N., Shen, B. (2018) Structural insights into the free-standing condensation enzyme SgcC5 catalyzing ester-bond formation in the biosynthesis of the enediyne antitumor antibiotic C-1027. Biochemistry 57:3278-3288.
(5)Wang, N., Rudolf, J. D., Dong, L.-B., Osipiuk, J., Hatzos-Skintges, C., Endres, M., Chang, C.-Y., Babnigg, G., Joachimiak, A., Phillips Jr., G. N., Shen, B. (2018). Discovery of a non-adenylating acyl-CoA ligase and restoring its adenylation activity. Nat. Chem. Biol. 14:730-737.
(6)Chang, C.-Y.; Yan, X.; Crnovcic, I.; Annaval, T.; Chang, C.; Nocek, B.; Rudolf, J. D.; Yang, D.; Hindra; Babnigg, G.; Joachimiak, A.; Phillips, G. N. Jr.; Shen, B. (2018) Resistance to enediyne antitumor antibiotics by sequestration. Cell Chem. Biol. 25:1075-1085.
(7)Annaval, T.; Han, L.; Rudolf, J. D.; Xie, G.; Yang, D.; Chang, C.-Y.; Ma, M.; Crnovcic, I.; Miller, M. D.; Soman, J.; Xu, W.; Phillips, G. N. Jr.; Shen, B. (2018) Biochemical and structural characterization of TtnD, a prenylated FMN-dependent decarboxylase from the tautomycetin biosynthetic pathway. ACS Chem. Biol. 13:2728-2738.
(8)Dong, L.-B.; Liu, Y.-C.; Cepeda, A. J.; Kalkreuter, E.; Deng, M.-R.; Rudolf, J. D.; Chang, C.; Joachimiak, A.; Phillips Jr, G. N.; Shen, B. (2019) Characterization and crystal structure of a nonheme diiron monooxygenase involved in platensimycin and platencin biosynthesis. J. Am. Chem. Soc. 141:12406-12412

Position Description

A Postdoctoral Associate is a direct pay individual in a Division 3 status. Postdoctoral Associates are appointed positions for the purpose of developing scholarly competence, working under the supervision of a TSRI Faculty member. These appointments are normally not renewed beyond a total of six years. Postdoctoral Associates cannot apply for external funding either as a Principal Investigator or Co-Principal Investigator without prior approval from a Departmental Chair. Postdoctoral Associates do not have consulting privileges.

Location: Florida
Additional Information:

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Physical Requirements:

This position works in a laboratory or vivarium environment. Further details of the physical requirements of established essential functions for this position will be addressed/discussed during the interview process.

Basic Qualifications:

Must possess a doctoral degree.

Preferred Qualifications:

Candidates with prior experience in natural product biosynthesis are especially encouraged to apply.

Compensation:
Job Closing Date:
Open Until Filled Yes
Job Posting Category Scientific & Technical
EEO Statement

The Scripps Research Institute is an Equal Opportunity Employer. All qualified applicants are encouraged to apply and will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other legally protected characteristic or status.

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